T-cell enumeration from dried blood spots by quantifying rearranged T-cell receptor-β genes
نویسندگان
چکیده
منابع مشابه
Chromatin Remodeling by the T Cell Receptor (Tcr)-β Gene Enhancer during Early T Cell Development
Gene targeting studies have shown that T cell receptor (TCR)-beta gene expression and recombination are inhibited after deletion of an enhancer (Ebeta) located at the 3' end of the approximately 500-kb TCR-beta locus. Using knockout mouse models, we have measured, at different regions throughout the TCR-beta locus, the effects of Ebeta deletion on molecular parameters believed to reflect epigen...
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The T cell repertoire is shaped by positive and negative selection of thymocytes through the interaction of alpha/beta-T cell receptors (TCR) with self-peptides bound to self-major histocompatibility complex (MHC) molecules. However, the involvement of specific TCR-peptide contacts in positive selection remains unclear. By fixing TCR-beta chains with a single rearranged TCR-beta irrelevant to t...
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We have examined the question of whether there is an additional checkpoint in T cell development that regulates T cell receptor (TCR)-beta expression in CD25+44- thymocytes by mechanisms that are independent of the pre-TCR. Our analysis in various mutant mice indicates that all changes in cytoplasmic TCR-beta expression can be accounted for by pre-TCR-dependent signal mediation, putting into qu...
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ژورنال
عنوان ژورنال: Journal of Immunological Methods
سال: 2010
ISSN: 0022-1759
DOI: 10.1016/j.jim.2010.01.008